Hot Start PCR Reagents and Kits
Promega GoTaq™ G2 Hot Start Polymerase
GoTaq™ G2 Hot Start Polymerase is supplied with 5X Green GoTaq™ Flexi Buffer, 5X Colorless GoTaq™ Flexi Buffer and 25mM MgCl2.
Promega GoTaq™ G2 Hot Start Colorless Master Mixes
GoTaq™ G2 Hot Start Master Mixes are ready-to-use mixes containing GoTaq™ G2 Hot Start Polymerase, buffer, dNTPs and optimized magnesium
Promega GoTaq™ Hot Start Polymerase: Colorless Master Mix, 2X
GoTaq™ Hot Start Master Mixes are all-in-one master mixes (just add template and primers) that contain the high-performance GoTaq™ DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until the reaction is heated at 94-95°C for two
Promega GoTaq™ MDx Hot Start Polymerase
GoTaq™ MDx Hot Start Polymerase contains GoTaq™ MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.
G-Biosciences Hot start High Fidelity DNA Polymerase 2X Master Mix
Hot start High Fidelity DNA Polymerase 2X Master Mix is used for PCR, Cloning and Diagnostics purposes.
Roche Diagnostics KAPA HiFi HotStart ReadyMix
KAPA HiFi HotStart ReadyMix is a 2X PCR master mix optimized for high-fidelity PCR amplification of DNA templates up to 5 kb. It contains KAPA HiFi HotStart DNA Polymerase, dNTPs, MgCl2 and stabilizers. The hot start polymerase allows room temperature reaction setup without non-specific amplification. KAPA HiFi HotStart ReadyMix delivers high yields and minimal bias for a wide range of targets, and is suitable for PCR applications including next-generation sequencing library construction, site-directed mutagenesis, and cloning of GC-rich targets.
Roche Diagnostics 2X KAPA2G Fast HotStart Genotyping Mix with dye, 1.25
2X KAPA2G Fast HotStart Genotyping Mix with dye, 1.25 *This item is non returnable.
Roche Diagnostics KAPA2G Fast PCR Kits - HotStart PCR Kit with Mg-free Buffer M, with dNTPs, 500 U
KAPA2G Fast PCR Kits - HotStart PCR Kit with Mg-free Buffer M, with dNTPs, 500 U. *This item is non returnable.
New England Biolabs, Inc. OneTaq Hot Start 2X Master Mix with GC Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
Roche Diagnostics KAPA HiFi HotStart ReadyMix PCR Kit (6.25 mL), 250 x 50 uL reactions
KAPA HiFi HotStart ReadyMix PCR Kit (6.25 mL), 250 x 50 uL reactions. *This item is non returnable.
New England Biolabs, Inc. NheI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence G^CTAGC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
101 BIO 1-Drop PCR Master Mix (with dye)
User can squeeze one drop of PCR Master mix into PCR tube/plate, add template and primers, and then can run PCR reaction. This PCR Master Mix contains Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. This unique Mix recipe makes the system very reliable, and tolerant to most of the PCR condition (concentration of the components). The Taq Polymerase in the PCR Master Mix is Hot Start polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. So no need to prepare the reactions on ice. The Polymerase is activated by a 10 minutes, 95°C incubation.
Sigma Aldrich Fine Chemicals Biosciences JumpStart REDTaq ReadyMix Reaction Mix | MFCD00166026 | 100 rxns
JumpStart REDTaq ReadyMix Reaction Mix | MFCD00166026 | 100 rxns